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elisa kits  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology elisa kits
    CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) <t>ELISA</t> quantification <t>of</t> <t>IL-1β,</t> IL-6, TNF-α, IL-17, and <t>MMP13</t> levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Elisa Kits, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+mmp+13/pmc13103408-183-16-30?v=Elabscience+Biotechnology
    Average 93 stars, based on 14 article reviews
    elisa kits - by Bioz Stars, 2026-07
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    Images

    1) Product Images from "Cycloastragenol attenuates osteoarthritis by restoring chondrocyte senescence via the NRF2/NF-κB signaling axis"

    Article Title: Cycloastragenol attenuates osteoarthritis by restoring chondrocyte senescence via the NRF2/NF-κB signaling axis

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-43064-z

    CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) ELISA quantification of IL-1β, IL-6, TNF-α, IL-17, and MMP13 levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) ELISA quantification of IL-1β, IL-6, TNF-α, IL-17, and MMP13 levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: CCK-8 Assay, Western Blot, Staining, Enzyme-linked Immunosorbent Assay



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    Image Search Results


    CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) ELISA quantification of IL-1β, IL-6, TNF-α, IL-17, and MMP13 levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Scientific Reports

    Article Title: Cycloastragenol attenuates osteoarthritis by restoring chondrocyte senescence via the NRF2/NF-κB signaling axis

    doi: 10.1038/s41598-026-43064-z

    Figure Lengend Snippet: CAG attenuates oxidative stress-induced chondrocyte senescence. ( A ) Chemical structure of CAG. ( B ) Effects of CAG treatment for 24 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( C ) Effects of CAG treatment for 48 h on the viability of chondrocytes were evaluated using the CCK-8 assay. n = 3. ( D – G ) Western blot analysis and quantification of senescence key markers (p53, CDKN1A, CDKN2A) in chondrocytes under CAG (50 μm) and TBHP (50 μm) treatments (See Supplementary Materials for original blots). n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001. ( H , I ) Representative images and quantitative analysis of β-galactosidase staining. n = 3, bar = 50 μm. ( J , K ) Representative images and quantitative analysis of EdU staining. n = 3, bar = 50 μm. ( L – P ) ELISA quantification of IL-1β, IL-6, TNF-α, IL-17, and MMP13 levels in chondrocytes under different treatments. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Levels of SASP-associated inflammatory and matrix-degrading components (IL-1β, IL-6, IL-17, TNF-α, MMP13) were quantified using commercial ELISA kits (IL-1β: E-EL-R0012; IL-6: E-EL-R0015; IL-17: E-EL-R0566; TNF-α: E-EL-R2856; MMP13: E-EL-R0045 all from Elabscience Biotechnology) according to the manufacturers’ instructions.

    Techniques: CCK-8 Assay, Western Blot, Staining, Enzyme-linked Immunosorbent Assay